Carbonated Calcium Phosphate Nanoparticle Synthesis

The suspension of caCP was prepared by wet chemical precipitation by dissolving calcium acetate (Ca(C₂H₃O₂)₂, Acros Organics BV, Geel, Belgium 99%) and disodium hydrogen phosphate (Na₂HPO₄, VWR International Ltd., Radnor, PA, USA, AnalaR). The Ca/P ratio was 5:3. The suspensions’ pH value was kept at 11 with a calculated content of sodium carbonate (anhydrous, VWR International Ltd., Radnor, PA, USA ≥99.5% ACS) to gain carbonated caCP nanoparticles. During the preparation process, the suspensions were stirred vigorously over 4 h at around 1400 rpm. The formed precipitates were cleaned thoroughly with distilled water and kept in an oven for 4 h at 150 °C. The resulting white nanopowders were gathered and prepared for further characterization and composite preparation.