Western Blot Analysis of Cellular Proteins

Total protein from cultured cells was extracted with RIPA buffer containing protease and phosphatase inhibitors. Equal amounts of protein were subjected to Western blotting as described previously.4 (link),22 (link),23 (link) The primary antibodies used were as follows: anti-THAP7 was purchased from Sigma-Aldrich, anti-p21 was obtained from Cell Signalling Technology (Cambridge, MA, USA), and anti-GAPDH was purchased from Bioworld Company (Nanjing, China).

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Chen C.P., Sang Y., Liu L., Feng Z.Q., Liang Z, & Pei X. (2019). THAP7 promotes cell proliferation by regulating the G1/S phase transition via epigenetically silencing p21 in lung adenocarcinoma. OncoTargets and therapy, 12, 5651-5660.

Publication 2019

anti-p21

Antibodies Buffer Cultured cells Gapdh Inhibitors Phosphatase Protease Protein Ripa

Corresponding Organization :

Other organizations : China Pharmaceutical University, Nanchang University, Third Hospital of Nanchang, Third Affiliated Hospital of Nanchang University, Jiangxi Provincial Cancer Hospital, Fifth Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University

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Variable analysis

independent variables

Protein extracted with RIPA buffer containing protease and phosphatase inhibitors

dependent variables

Protein expression levels of THAP7, p21, and GAPDH

control variables

Equal amounts of protein subjected to Western blotting

controls

Positive control: None mentioned
Negative control: None mentioned

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