Lung Cancer Cell Line Cultivation

Lung cancer cell lines (A549, H2009, H1819, Calu3, H23, H1993, H226, H460, and H1299) and the normal bronchial epithelial cell line (BEAS2B) were obtained from the American Type Culture Collection, as previously described [7 (link)]. The cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA) or RPMI-1640 medium (Thermo Fisher Scientific), supplemented with 1% penicillin–streptomycin and 10% fetal bovine serum.

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Arimura K., Kammer M., Rahman S.M., Sheau-Chiann C., Zhao S., Heidi C., Eisenberg R., Zou Y., Antic S., Richmond B., Tagaya E., Grogan E., Massion P, & Maldonado F. (2024). Elucidating the role of EPPK1 in lung adenocarcinoma development. BMC Cancer, 24, 441.

Publication 2024

H2009 H1819 Calu3 H1993 H1299 BEAS2B DMEM RPMI-1640 medium

Corresponding Organization :

Other organizations : Vanderbilt University Medical Center, Tokyo Women's Medical University

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Variable analysis

independent variables

Cell lines (A549, H2009, H1819, Calu3, H23, H1993, H226, H460, H1299, and BEAS2B)

dependent variables

Not explicitly mentioned

control variables

Culture medium (Dulbecco's modified Eagle's medium (DMEM) or RPMI-1640 medium)
Penicillin–streptomycin (1%)
Fetal bovine serum (10%)

controls

No positive or negative controls were explicitly mentioned

Annotations

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