Immunoblot Analysis of Cellular Signaling

Immunoblots were performed based on methods described in a previous paper [13 (link)]. The following antibodies were used: anti-NRF2 (GTX103322, GeneTex, Irvine, CA, USA), anti-GAPDH (60004-1g, Proteintech, Rosemont, IL, USA), anti-IκB alpha (12045-R116, Sino Biological, BDA, Beijing, China), anti-phospho- IκB alpha (#9246, Cell Signaling Technology, MA, USA), anti-alpha Tubulin (11224-1-AP, Proteintech, Rosemont, IL, USA), and anti-Lamin B1 (66095-1-Ig, Proteintech, Rosemont, IL, USA). Nuclear and cytoplasmic proteins were collected using EPIXTRACT Nuclear Protein Isolation Kit according to the manufacturer’s instructions.

Free full text: Click here

Wang S.H., Chen Y.S., Lai K.H., Lu C.K., Chang H.S., Wu H.C., Yen F.L., Chen L.Y., Lee J.C, & Yen C.H. (2022). Prinsepiae Nux Extract Activates NRF2 Activity and Protects UVB-Induced Damage in Keratinocyte. Antioxidants, 11(9), 1755.

Publication 2022

anti-NRF2 (GTX103322, anti-GAPDH (60004-1g, anti-alpha Tubulin (11224-1-AP,

Alpha tubulin Anti b1 Antibodies Biological Cytoplasmic Gapdh Immunoblots Isolation Iκb alpha Lamin Lamin b1 Nrf2 Nuclear protein Proteins Sino

Corresponding Organization : National Health Research Institutes

Other organizations : Taipei Medical University, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, National Sun Yat-sen University

Top 5 similar protocols

Variable analysis

independent variables

Antibodies used for immunoblotting

dependent variables

Levels of NRF2, GAPDH, IκB alpha, phospho-IκB alpha, alpha Tubulin, and Lamin B1 proteins

control variables

Nuclear and cytoplasmic protein samples collected using EPIXTRACT Nuclear Protein Isolation Kit

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!