Histological Evaluation of Cardiac Fibrosis

Heart tissues from each mouse were fixed in 10% formalin, paraffin-embedded, and sectioned in 7 μm sections for hematoxylin-eosin staining following standard protocols, as previously described [50 (link)]. After scanning the slides with GTX450 (Leica biosystems) and conducting the initial histologic evaluation, histochemistry with Masson’s trichrome was performed (Ventana system; Roche). Histochemistry was performed following the manufacturer’s instructions. Fibrosis area was determined using ImageJ version 1.54. An expert pathologist performed the histopathological analyses of the heart valves following a blinded protocol.

Free full text: Click here

Herrera-Martínez A.D., Fuentes-Fayos A.C., Sanchez-Sanchez R., Montero A.J., Sarmento-Cabral A., Gálvez-Moreno M.A., Gahete M.D, & Luque R.M. (2024). Does Telotristat Have a Role in Preventing Carcinoid Heart Disease?. International Journal of Molecular Sciences, 25(4), 2036.

Publication 2024

Ventana system

Corresponding Organization : Hospital Universitario Reina Sofía

Other organizations : University of Córdoba, Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Fibrosis area in heart tissues

control variables

Tissue preparation: Heart tissues were fixed in 10% formalin, paraffin-embedded, and sectioned in 7 μm sections
Staining: Hematoxylin-eosin staining and Masson's trichrome staining were performed following standard protocols
Imaging: Slides were scanned with GTX450 (Leica biosystems)

controls

Positive control: None mentioned
Negative control: None mentioned
Blinded protocol: An expert pathologist performed the histopathological analyses of the heart valves following a blinded protocol

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!