Protein Extraction and Western Blot Analysis

RIPA lysate buffer (Servicebio, Wuhan, China) was used to separate protein from gastric tissue, and the protein concentration was measured by a BCA kit (Servicebio, Wuhan, China). Prepared protein samples were separated by gel electrophoresis and transferred to solid phase carrier (PVDF) membranes, which were incubated with special antibodies and washed with TBST. After that, the membranes were soaked in ECL chemiluminescence solution to detect the target protein blots which were visualized by ImageQuant LAS4000mini system (GE, Boston, MA, USA). During the whole process, β-actin was treated as a control protein [22 (link),23 (link)].