Detection of Enteric Pathogens in Stool

A single, fresh, whole stool specimen was collected from cases and controls at enrollment for the recovery of potential enteropathogens. Various specific growth media were used for detecting the bacterial pathogens. Up to 3 colonies with the appearance of E. coli on MacConkey agar were selected from each sample and tested using multiplex polymerase chain reaction (PCR) for enterotoxigenic E coli (ETEC) (heat-labile [LT] and heat-stable [ST] enterotoxins), enteropathogenic E. coli (EPEC) (eae and bfpA), and EAEC (aaiC and aatA). Any colonies that were positive for either aaiC (chromosomally encoded) or aatA (encoded on the pAA plasmid) were considered EAEC for the purposes of this analysis.

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Boisen N., Scheutz F., Rasko D.A., Redman J.C., Persson S., Simon J., Kotloff K.L., Levine M.M., Sow S., Tamboura B., Toure A., Malle D., Panchalingam S., Krogfelt K.A, & Nataro J.P. (2011). Genomic Characterization of Enteroaggregative Escherichia coli From Children in Mali. The Journal of Infectious Diseases, 205(3), 431-444.

Publication 2011

Agar Bacterial E coli Enterotoxigenic e coli Enterotoxins Epec Growth media Multiplex polymerase chain reaction Pathogens Plasmid Stool

Corresponding Organization :

Other organizations : University of Virginia, Statens Serum Institut, University of Maryland, Baltimore, Centre Pour le Développement des Vaccins-Mali

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Protocol cited in 14 other protocols

Variable analysis

independent variables

Type of growth media used for detecting bacterial pathogens

dependent variables

Recovery of potential enteropathogens
Presence of enterotoxigenic E. coli (ETEC) (heat-labile [LT] and heat-stable [ST] enterotoxins)
Presence of enteropathogenic E. coli (EPEC) (eae and bfpA)
Presence of enteroaggregative E. coli (EAEC) (aaiC and aatA)

control variables

Single, fresh, whole stool specimen collected from cases and controls at enrollment

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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