Microarray Analysis of Mouse Transcriptome

Microarray analysis was performed with the GeneChip Mouse Gene 2.0 ST Array (Affymetrix, Santa Clara, CA, USA) with targets derived from total RNA from NAC. Approaches were identical to those recently described (Eisinger et al., 2014 (link)) and included the Ambion GeneChip WT Expression Kit (Ambion, Austin, TX, USA), the Affymetrix WT Terminal Labeling Kit (Affymetrix), and an Affymetrix GC3000 G7 Scanner. Data were extracted and processed in the Affymetrix Command Console v. 3.1.1.1.229 and cDNA synthesis, fragmentation, labeling, array hybridization, staining, and scanning were performed by the Gene Expression Center at the University of Wisconsin-Madison as in previous studies (Eisinger et al., 2013b (link), 2014 (link); Driessen et al., 2014a (link)).