Quantifying Cellular Iron, ROS, and GSH

The production of the cellular labile iron pool (LIP) was determined through assessing intracellular iron concentration by using an iron assay kit (Abcam, ab83366). In addition, ROS (cat. no. E004), and GSH (cat. no. A006) was determined using commercial ELISA kits (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturers’ instructions.18 (link)

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Yin X., Yang Q., Li H., Kang Y, & Li Z. (2023). Vancomycin Induced Ferroptosis in Renal Injury Through the Inactivation of Recombinant Glutathione Peroxidase 4 and the Accumulation of Peroxides. Drug Design, Development and Therapy, 17, 283-295.

Publication 2023

ab83366 ELISA kits

Assay Cellular Elisa Intracellular Iron

Corresponding Organization :

Other organizations : Shanghai Children's Hospital, Shanghai Jiao Tong University, Hunan Children's Hospital

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Variable analysis

independent variables

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dependent variables

Intracellular iron concentration

control variables

None explicitly mentioned

controls

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