Oral CCL11 Sensitization and OVA Challenge

The oral treatment with a recombinant CCL11 (R&D Systems, Minneapolis, MN, USA) was performed as previously described with modifications (27 (link)). Briefly, groups of sensitized mice were deprived of food for 2 h, followed by intragastric administration of an isotonic bicarbonate solution (8 parts HBSS and 2 parts 7.5% sodium bicarbonate) to neutralize stomach acidity. Individual mice were gavaged with a 0.1 ml PBS solution (pH 7.2) containing 1 µg of recombinant CCL11 and 30 min later, were gavaged with 50 mg of OVA in 0.5 ml of PBS.

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Kim E., Lembert M., Fallata G.M., Rowe J.C., Martin T.L., Satoskar A.R., Reo N.V., Paliy O., Cormet-Boyaka E, & Boyaka P.N. (2018). Intestinal Epithelial Cells Regulate Gut Eotaxin Responses and Severity of Allergy. Frontiers in Immunology, 9, 1692.

Publication 2018

recombinant CCL11

Bicarbonate Ccl11 Food Hbss Isotonic solution Mice Oral treatment Sodium bicarbonate Stomach acidity

Corresponding Organization : The Ohio State University

Other organizations : Wright State University

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Variable analysis

independent variables

Dose of recombinant CCL11 (1 µg)

dependent variables

Allergic response to OVA challenge

control variables

Time of food deprivation (2 h)
Composition of intragastric solution (8 parts HBSS and 2 parts 7.5% sodium bicarbonate)
Dose of OVA challenge (50 mg in 0.5 ml of PBS)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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