Nesf-1 Protein Expression Analysis

Bottlenose dolphin pancreas and rat testis samples were processed as previously described (Gatta et al., 2014 (link)). The same blot membrane was stripped and re-probed against β-actin (A5060, Sigma, Sant Louis, MO, United States), used as internal marker. In the specific step, primary polyclonal antibody raised in rabbit against Nesf-1 (1:2000, Phoenix Pharmaceuticals, cat. No. H-003-24) was incubated ON at 4 °C. This was followed by incubation with the secondary goat anti-rabbit IgG (1:10000, Santa Cruz Biotechnology Inc., cat. No. sc-2004) for 1 h, RT.

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Gatta C., De Felice E., D’Angelo L., Maruccio L., Leggieri A., Lucini C., Palladino A., Paolucci M., Scocco P., Varricchio E, & de Girolamo P. (2018). The Case Study of Nesfatin-1 in the Pancreas of Tursiops truncatus. Frontiers in Physiology, 9, 1845.

Publication 2018

β-actin Nesf-1 secondary goat anti-rabbit IgG

Actin Anti igg Antibody Bottlenose dolphin Goat Membrane Pancreas Pharmaceuticals Rabbit Testis

Corresponding Organization : Stazione Zoologica Anton Dohrn

Other organizations : Center for Advanced Biomaterials for Healthcare, Italian Institute of Technology, University of Sannio

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Variable analysis

independent variables

Bottlenose dolphin pancreas samples
Rat testis samples

dependent variables

Nesf-1 protein expression

control variables

β-actin (used as internal marker)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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