Eosinophil Quantification in Skin Tissues

All skin tissues were fixed in 4% paraformaldehyde and then buried in paraffin blocks. The tissues were sliced into 5 μm sections for pathological analysis with hematoxylin-eosin staining and for eosinophils counts by Chromotrope-2R staining [54 (link)]. The stained slides were scanned with Olympus VS120 S6 Slide scanner (OLYMPUS, Tokyo, Japan). The measurements were carried out using ImagePro Plus 6.0. The number of eosinophils in scanned files was counted from 9 consecutive square areas (each at 0.09 mm² area) for each sample [54 (link)].

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Gu X., Ge Y., Wang Y., Huang C., Yang G., Xie Y., Xu J., He R., Zhong Z., Yang D., He Z, & Peng X. (2023). Macrophage Migration Inhibitory Factor in Psoroptes ovis: Molecular Characterization and Potential Role in Eosinophil Accumulation of Skin in Rabbit and Its Implication in the Host–Parasite Interaction. International Journal of Molecular Sciences, 24(6), 5985.

Publication 2023

VS120 S6 Slide scanner

Chromotrope 2r Eosin Eosinophils Paraffin Paraformaldehyde Skin tissues Tissues

Corresponding Organization : Sichuan Agricultural University

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Variable analysis

independent variables

Tissue samples

dependent variables

Eosinophil counts

control variables

Tissue fixation in 4% paraformaldehyde
Paraffin embedding of tissue samples
Tissue sectioning at 5 μm thickness
Hematoxylin-eosin staining
Chromotrope-2R staining for eosinophil counts
Slide scanning with Olympus VS120 S6 Slide scanner
Image analysis using ImagePro Plus 6.0

controls

No positive or negative controls were explicitly mentioned in the provided information.

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