Cultivation of SKOV-3 Ovarian Cancer Cells

The human ovarian adenocarcinoma cell line SKOV-3 (ATCC HTB-77) was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultivated in Dulbecco's modified Eagle's medium (DMEM; Gibco, Paisley, UK) supplemented with 10% heat-inactivated fetal calf serum (FCS; Gibco), 2 mM L-glutamine (Gibco) and penicillin 100 IU/ml and 100 µg/ml streptomycin (Gibco) at 37°C in a humidified atmosphere with 5% CO₂. Cells were grown in 75 cm² cell culture flasks (Costar, Cambridge, MA, USA) and subcultured twice a week. Suspensions of the cells were obtained by washing the cells twice with 10% phosphate buffered saline (PBS; Dulbecco's tablets, Oxoid Limited, Hampshire, UK) and incubating the cell cultures with Trypsin EDTA (Gibco). Thereafter, the cells were washed in growth medium, resuspended or snap-frozen for later thawing and reuse [21] (link).