Fatty Acid Composition Analysis

Fasting blood samples were collected at each of the 9 clinic visits during the 12-mo intervention, from which plasma phosphatidylcholine, platelets, and MNCs were isolated and analyzed for FA composition. The preparation and analysis of blood samples has been described previously (15 (link)). Briefly, FAs were analyzed by GC, performed on a Hewlett Packard 6890 gas chromatograph fitted with a BPX-70 column (30 m × 0.22 mm × 0.25 μm). The instrument was controlled by and data were collected using HPChemStation (Hewlett Packard). FAMEs were identified by comparison of retention times with those of authentic standards run previously.