Tissue collection and gene expression analysis

The head (without antennae), thoraxes (without legs), abdomens were dissected from 15 virgin 1-day-old of females or males, respectively. These tissues were immediately transferred into 1.5 mL RNA-free tube, super-cooled via liquid nitrogen, and then stored at − 80 °C freezer. These tissues were used for RNA extraction with RNAiso Plus (TAKARA, 9109, Dalian, China) and then cDNA synthesis with A Prime Script RT reagent Kit with gDNA Eraser (TAKARA, RR047, Dalian, China). The quantitative PCR reactions were conducted on an ABI QuantStudioTM 6 Flex system (Thermo Fisher Scientific, Massachusetts, USA). The PCR reaction was performed with each reaction was performed with Green Premix Ex Taq II Kit (TAKARA, RR820A, Dalian, China) and prepared as introduced^{42 (link)}. The expression level of target gene was normalized with reference gene TUB1 (α-tubulin) and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) via 2^-∆∆CT method according to our previous works^{39 ,42 (link)}. The primers used in this research were listed in the Table S1.