Cytotoxicity Assay for Anti-Cancer Drugs

Cells (3 × 10³) were plated in each well of a 96-well plate (Greiner bio-one 655090) with increasing concentrations of cisplatin (Cayman Chemical, 13119) dissolved in DMSO. DMSO concentrations were identical for all conditions. Each condition of cisplatin concentrations was performed in triplicate. After 48 or 72 hours, plates were read using a Cell Titer Blue reagent (Promega, G8081). IC50 calculations were obtained with Calcusyn software (Biosoft). Gemcitabine (Caymen Chemical, 11690) and carboplatin (TCI, C2043) experiments were done similarly. For experiments involving exosomes, 350 million exosomes were used per reaction. For assays with inhibitors, 1 µM of MK-2206 or 5 µM of LiCl was utilized based on Meng et al.^{50 (link)} and Coghlan et al.^{51 (link)}, respectively.