Isolation of CD11b+ immune cells

Minced spleens and lymph nodes were passed through a 40μm strainer to get a single-cell suspension as described previously [16 (link), 47 (link)]. After lysis of red blood cells, the cell suspension was incubated with CD11b microbeads mouse / human (130-049-601, Miltenyi Biotec) for 15 minutes at 4°C. CD11b-positive cells were eluted from the beads using MACS^® separation columns (130-042-201, Miltenyi Biotec) according to the manufacturer’s instructions and collected for subsequent RNA extraction.

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Li X., Singh K., Luo Z., Mejia-Cordova M., Jamalpour M., Lindahl B., Zhang G., Sandler S, & Welsh M. (2018). Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas. Oncotarget, 9(27), 18720-18733.

Publication 2018

CD11b microbeads mouse / human MACS® separation columns

Cd11b Cells Human Lymph nodes Microbeads Mouse Red blood cells

Corresponding Organization :

Other organizations : Soochow University, Uppsala University

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Variable analysis

independent variables

Passing minced spleens and lymph nodes through a 40μm strainer
Incubating the cell suspension with CD11b microbeads mouse / human for 15 minutes at 4°C
Eluting CD11b-positive cells from the beads using MACS® separation columns

dependent variables

RNA extracted from the collected CD11b-positive cells

control variables

Use of a 40μm strainer to get a single-cell suspension
Lysis of red blood cells
Use of MACS® separation columns according to the manufacturer's instructions

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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