Immunostaining of Epithelial Markers

Staining was performed as previously described (Mitsiadis et al., 2017 (link); Jimenez-Rojo et al., 2019 (link)). Primary antibodies used: polyclonal rabbit anti-Keratin14 (dilution 1:100; Poly19053, Biolegend), polyclonal Goat anti-E-Cadherin (dilution 1:200, AF748, R&D Systems), rat anti-BrdU (dilution 1:50; OBT0300, AbD Serotec), polyclonal Rabbit anti-GFP (dilution 1:100; A11122; Invitrogen). The antibodies were incubated at 4°C overnight. Secondary antibodies used: Goat anti-Rabbit IgG 488, Alexa Fluor Plus 488 (dilution 1:1000; A32731, Invitrogen), Donkey anti-Goat IgG 568, Alexa Fluor Plus 568 (dilution 1:1000; Invitrogen); Goat anti-Rat 546, Alexa Fluor Plus 546 (dilution 1:1000; Invitrogen), Donkey anti-Rabbit IgG 488, Alexa Fluor 488 (dilution 1:1000; A21206: Invitrogen). These antibodies were incubated for 1 h at room temperature (RT).