Bio MTA+, MTA Cem, Biodentine and NeoMTA were tested in this study. The composition of the test materials and their manufacturers are provided in Table 1 . Each material was mixed as per the instructions outlined by the manufacturer in a laminar flow cabin under aseptic conditions. Each mixture was then inserted into a custom-made presterilized teflon mold with a diameter of 8 mm and a thickness of 2.0 mm and left undisturbed for four hours. Fifteen sample discs for each group were obtained. Upon complete setting, the surfaces of the specimens were exposed to 30 min of ultraviolet light to ensure sterility [21 (link)]. All discs were immersed in 50 ml Falcon tubes filled with 25 ml of DMEM (Thermo Fisher Scientific 31,885,023, MA, USA) and stored in an incubator at 37 °C for 24 h. The extracts of the materials were filtered with 0.22-µm pore size filter (Sartorius AG, Germany). The stock extracts were kept at -20 °C until experiments.
Manufacturers and compositions of CSCs used in the study
| Material | Manufacturer | Batch No | Composition |
|---|---|---|---|
| Bio MTA+ | Cerkamed, Poland | 2811221 | Powder: Calcium oxide, hydroxyapatite, oxides of: silicon, iron, aluminum, sodium, potassium, bismuth, magnesium, zirconium; calcium phosphate. Liquid: Purified water, calcium catalyst. |
| MTA Cem | NexoBio, Chungcheongbuk-do, South Korea | MC180601 | Powder: Tricalcium silicate, dicalcium silicate, tricalcium aluminate, bismuth oxide Liquid: Distilled water |
| Biodentine | Septodont, Saint-Maur-des-Fosses, France | B24589 | Powder: Tricalcium silicate, Zirconium oxide, Calcium carbonate, Calcium chloride, polymer Liquid: Aqueous solution of calcium chloride and polycarboxylate. |
| NeoMTA | Nusmile Inc., Houston, TX; USA | 2022051801 | Powder: Tricalcium silicate, dicalcium silicate, tantalite, and minor amounts of calcium sulfate and tricalcium aluminate Liquid: water and proprietary polymers |
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