Differentiation of THP-1 Cells into Macrophages

To generate THP-1 derived macrophages (TDM), THP-1 cells (in 0.4 μm transwell insert for 6-well plate) were treated with 10 ng/ml phorbol 12-myristate 13-acetate (PMA) for 24 hours. Then, the cells were cultured with 25 ng/ml IL-4 or LPS for another 48 hours to generate THP-1-derived macrophages. Meanwhile, THP-1 cells were treated with 10 ng/ml phorbol 12-myristate 13-acetate (PMA) for 24 hours before further treatment (B. thetaiotaomicron, heat killed B. thetaiotaomicron, B. thetaiotaomicron culture medium or acetic acid).

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Ma H., Yang L., Liang Y., Liu F., Hu J., Zhang R., Li Y., Yuan L, & Feng F. (2024). B. thetaiotaomicron-derived acetic acid modulate immune microenvironment and tumor growth in hepatocellular carcinoma. Gut Microbes, 16(1), 2297846.

Publication 2024

Corresponding Organization : Quzhou City People's Hospital

Other organizations : Shanghai First Maternity and Infant Hospital

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Variable analysis

independent variables

Treatment with 10 ng/ml phorbol 12-myristate 13-acetate (PMA) for 24 hours
Treatment with 25 ng/ml IL-4 or LPS for 48 hours
Treatment with B. thetaiotaomicron, heat killed B. thetaiotaomicron, B. thetaiotaomicron culture medium or acetic acid

dependent variables

Generation of THP-1-derived macrophages

control variables

THP-1 cells (in 0.4 μm transwell insert for 6-well plate)

controls

Positive control: Treatment with 25 ng/ml IL-4
Negative control: Treatment with 25 ng/ml LPS

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