Protein Extraction and Western Blotting

Protein extraction and western blotting analysis were performed using previously standard procedures (17 (link)). The following antibodies were used for western blotting: anti-E-cadherin antibody(#20874-1-AP Proteintech, China), anti-N-cadherin antibody(#22018-1-AP Proteintech, China), anti-ZEB1 antibody(#66279-1-Ig Proteintech, China), anti-Vimentin antibody(10366-1-AP Proteintech, China), anti-SOX2 antibody(#11064-1-AP Proteintech, China), anti-GAPDH antibody(#10494-1-AP Proteintech, China), and anti-PD-L1 antibody(#66248-1-Ig Proteintech, China).

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Mao J., Tao Y., Wang K., Sun H., Zhang M., Jin L, & Pan Y. (2024). Identification of hub genes within the CCL18 signaling pathway in hepatocellular carcinoma through bioinformatics analysis. Frontiers in Oncology, 14, 1371990.

Publication 2024

anti-E-cadherin antibody anti-N-cadherin antibody anti-ZEB1 antibody anti-Vimentin antibody anti-SOX2 antibody anti-GAPDH antibody

Corresponding Organization : China Pharmaceutical University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein expression levels of E-cadherin, N-cadherin, ZEB1, Vimentin, SOX2, GAPDH, and PD-L1

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input.

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