Roots of pUBI::OsSCR106-EGFP and pOsSCR106::OsSCR106-EGFP were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 2 h, washed with dH2O and incubated in ClearSee solution (Kurihara et al., 2015 (link)) for ~2 weeks, and then imaged with the Zeiss LSM710 inverted microscope.
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