Quantifying DHME-Induced Apoptosis in CRC

DHME-induced apoptosis was quantitatively evaluated by flow cytometry analysis to identify the levels of annexin V-stained (apoptotic) cells on the Muse Cell Analyzer, using Muse annexin V and a Dead Cell Assay Kit (Millipore; Burlington, MA, USA), in accordance with the procedures reported previously [35 (link)]. To elucidate the significance of apoptosis induction to DHME-induced cytotoxicity, CRC cells were pre-treated for 1 h with 50 μM of z-VAD-fmk to block apoptosis, followed by 24 h incubation with DHME for subsequent apoptosis analysis.

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Fan H.C., Hsieh Y.C., Li L.H., Chang C.C., Janoušková K., Ramani M.V., Subbaraju G.V., Cheng K.T, & Chang C.C. (2020). Dehydroxyhispolon Methyl Ether, A Hispolon Derivative, Inhibits WNT/β-Catenin Signaling to Elicit Human Colorectal Carcinoma Cell Apoptosis. International Journal of Molecular Sciences, 21(22), 8839.

Publication 2020

Muse Cell Analyzer Dead Cell Assay Kit

Annexin v Apoptosis Assay Block Cells Cytotoxicity Flow cytometry Muse Z vad fmk

Corresponding Organization : China Medical University

Other organizations : Tungs' Taichung MetroHarbor Hospital, Kang-Ning Junior College of Medical Care and Management, University of Chemistry and Technology, Andhra University

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Variable analysis

independent variables

DHME (concentration not specified)
Z-VAD-fmk (50 μM)

dependent variables

Levels of annexin V-stained (apoptotic) cells

control variables

Incubation time with DHME (24 h)
Pre-treatment time with z-VAD-fmk (1 h)

controls

Positive control: Not specified
Negative control: Not specified

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