Immunofluorescence Staining and Imaging

Immunofluorescence was performed as previously described (Fu et al., 2017 (link)). Antibodies used for staining are as followed: anti-VP1 antibody (ab36367, Abcam), Flag (F1804, Sigma), DDX6 (A300-460A, Belthy-Lab). The secondary antibodies are: Alexa Fluor goat anti-mouse-594 IgG (h+L) (1:1000 dilution), Alexa Fluor goat anti-rabbit-594 IgG (h+L) (1:1000 dilution) and Alexa Fluor goat anti-rabbit-488 IgG (h+L) (1:1000 dilution, Life Technologies). All images were taken using the Olympus FV3000 confocal microscope (Tokyo, Japan).

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Zhang R., Cheng M., Liu B., Yuan M., Chen D., Wang Y, & Wu Z. (2021). DEAD-Box Helicase DDX6 Facilitated RIG-I-Mediated Type-I Interferon Response to EV71 Infection. Frontiers in Cellular and Infection Microbiology, 11, 725392.

Publication 2021

ab36367 FV3000 confocal microscope

Anti antibody Anti igg Antibodies Confocal microscope Ddx6 Dilution Goat Immunofluorescence Mouse Rabbit

Corresponding Organization : Ningxia University

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Variable analysis

independent variables

Anti-VP1 antibody (ab36367, Abcam)
Flag (F1804, Sigma)
DDX6 (A300-460A, Belthy-Lab)

dependent variables

Immunofluorescence staining

control variables

Experimental protocol as previously described (Fu et al., 2017)
Dilution of secondary antibodies (1:1000)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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