Apoptosis and Mitochondrial Potential Analysis

Flow cytometric analyzed of apoptosis were used the Annexin V-FITC/PI Apoptosis Detection Kit (KeyGEN, China), and was presented as protocol described (25 (link)). The cell’s inner mitochondrial membrane potential (Δψm) was detected by flow cytometric using MitoScreen JC-1 staining kit (BD) (26 (link)). Briefly, cells were dissociated with trypsin and resuspended at 1 × 10⁶ cells/ml in Assay Buffer, and then incubated at 37°C for 15 min with 10 μl/ml JC-1. Before analyzed by flow cytometer, cells were washed twice by Assay Buffer. Flow cytometry data were analyzed using FlowJo 7.6 software (TreeStar Inc., USA) as previously described (27 (link)).