ABMMCs were isolated and cultured using previously described methods [26 (link)]. Mandibles without molars or soft tissue were obtained from 4-week-old Wistar rats, and then digested with 3 mg/mL collagenase I (Solarbio) and 4 mg/mL dispase II (Roche) at 37 °C for 1 h. Cells were then collected and cultured in primary medium containing α-minimal essential medium (α-MEM; BasalMedia), 15% fetal bovine serum (FBS; Lonsa Science Srl), and 10,000 U/mL penicillin-streptomycin (Biosharp). The ABMMCs used in this study were 3–5 generations.
Flow cytometry was used to identify the phenotype of ABMMCs. In brief, ABMMCs were resuspended with phosphate-buffered saline (PBS; BasalMedia). Then, CD44, CD31 (BD; Biosciences), CD90, and CD45 (Elabscience) antibodies were applied to detect the phenotype of ABMMCs as directed by the manufacturer.
Flow cytometry was used to identify the phenotype of ABMMCs. In brief, ABMMCs were resuspended with phosphate-buffered saline (PBS; BasalMedia). Then, CD44, CD31 (BD; Biosciences), CD90, and CD45 (Elabscience) antibodies were applied to detect the phenotype of ABMMCs as directed by the manufacturer.
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