Western Blot Antibody Profiling for HTLV-1 Proteins

Primary antibodies against proteins of interest used in Western blot assays were: α-Tax (Tax antibodies 169, 170, and 171 monoclonal mouse (generous gift of Dr. Scott Gitlin, University of Michigan) [35 (link), 54 (link)]; α-p19 (Santa Cruz Biotechnology, sc-1665); α-gp61/46 (NIH AIDS Reagent Program Cat. # 1578); LC3-1/LC3-II (Santa Cruz Biotechnology, Cat. # SC-398822); p62 autophagy marker (Cell Signaling Inc., Cat. # 5114S); α-CD45 (Santa Cruz Biotechnology, Cat. # SC-1123); α-CD43 (Santa Cruz Biotechnology, Cat. # SC-70682); α-ICAM-1 (Santa Cruz Biotechnology, Cat. # SC-8439); α-LFA-1 (Santa Cruz Biotechnology, Cat. # SC-374172); α-CD63 (Santa Cruz Biotechnology, Cat. # SC-365604); α-CD63 tetraspanin marker (SBI System Biosciences, Cat. # EXOAB-CD63A-1); α-histone H2B (Cell Signaling Technology, Cat. # 12,364); α-histone H2A (Santa Cruz Biotechnology, Inc., Cat. # SC-10807 and abcam, Cat. # ab18255); α-histone H3 (Santa Cruz Biotechnology, Inc., Cat. # SC-10809); α-histone H4 (Cell Signaling Technology, Cat. # 2592); α-histone H1 (GeneTex, Cat. # GTX114462); IL-8 (Santa Cruz Biotechnology, Cat. # SC-376750); IL-6 (Santa Cruz Biotechnology, Cat. # SC-28343); RANTES (Santa Cruz Biotechnology, Cat. # SC-514019); GAPDH (Santa Cruz Biotechnology, Cat. # SC-48166); and α-Actin (Abcam; ab469900).

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Pinto D.O., Al Sharif S., Mensah G., Cowen M., Khatkar P., Erickson J., Branscome H., Lattanze T., DeMarino C., Alem F., Magni R., Zhou W., Alais S., Dutartre H., El-Hage N., Mahieux R., Liotta L.A, & Kashanchi F. (2021). Extracellular vesicles from HTLV-1 infected cells modulate target cells and viral spread. Retrovirology, 18, 6.

Publication 2021

α-CD45 α-ICAM-1 α-CD63 IL-6 RANTES GAPDH α-Actin

Actin Aids Antibodies Autophagy Cat 1 Cd43 Gapdh Histone h1 Histone h2a Histone h2b Histone h3 Histone h4 Icam 1 Lfa 1 Mouse Proteins Rantes Tetraspanin Western blot assays

Corresponding Organization :

Other organizations : George Mason University, Inserm, École Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Florida International University

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Variable analysis

independent variables

Primary antibodies against proteins of interest used in Western blot assays

dependent variables

Protein expression levels measured by Western blot

control variables

GAPDH
Actin

positive controls

Tax antibodies 169, 170, and 171 monoclonal mouse (generous gift of Dr. Scott Gitlin, University of Michigan)

negative controls

Not explicitly mentioned

Annotations

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