Comprehensive Hormonal Assessment in IVF

Blood tests were performed on cycle day 2 and the hCG trigger day. The concentrations of serum AMH in all subjects were measured before the start of IVF treatment. The concentrations of FSH, LH, E2, and progesterone were quantitatively determined by chemiluminescence assay (Abbott Biologicals B.V., Olst, Overijssel, The Netherlands) as described in our previous work [32 (link)]. We defined the detection limit as follows: FSH = 0.06 mIU/mL, LH = 0.09 mIU/mL, E2 = 10 pg/mL, and P = 0.1 ng/mL. For AMH measurement, all samples were immediately assayed with a commercial ELISA kit (AMH Gen II assay, Beckman Coulter, Brea, CA) according to the manufacturer’s instructions. The intra- and inter-assay coefficients of variation did not exceed 6% and 16%, respectively.