The GST assay reaction mixture contained in 1 ml total volume of 0.1 M K-phosphate buffer, pH 6.5, 1 mM 1-chloro-2,4-dinitrobenzene (CDNB), 1 mM GSH, and enzyme solution. The increase in absorbance was monitored at 340 nm for 3 min at 25 °C. The product extinction coefficient was considered as 9.6 mM−1 cm−1. One GST unit is equivalent to the enzyme amount which conjugates 1 μmole CDNB in 1 min [19 (link)].
Free full text: Click here