Cryptosporidium Detection in HIV/AIDS Patients

A cross-sectional study was conducted. Faecal sampling was carried out from 2016 to 2018 and obtained from 350 patients with an HIV/AIDS positive status associated with diarrhoea attending inpatient (hospitalisation) and outpatient care units at El Hadi Flici (ex El- Kettar) hospital, Alger city, Algeria. After obtaining informed consent, patients completed a comprehensive questionnaire on age, sex, contact with animals (pets and farm animals) and drinking water sources. Clinical characteristics, including diarrhoea, weight loss, vomiting, abdominal pain and nausea, types of HAART drug regimens and laboratory characteristics, including blood CD4+ T-cell counts, were recorded by the physicians in charge. Cryptosporidium microscopy-based screening was performed in El Hadi Flici Ex El- Kettar hospital, Alger city, Algeria. All specimens were smeared onto glass slides, stained using the modified Ziehl Nielsen (mZN) and auramine techniques [15 (link)] and examined using light (1000×) and fluorescence (100× and 400×) microscopy, respectively (Figure 1). A sample was considered Cryptosporidium-positive if typical 4–6 μm diameter oocysts were visible. Positive samples were transferred to the Centre National de Référence–Laboratoire Expert crypyosporidioses (CNR-LE) (Rouen University Hospital, France) for molecular analysis.

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Semmani M., Costa D., Achour N., Cherchar M., Ziane H., Mouhajir A., Villier V., Adjmi Hamoudi H., Favennec L, & Razakandrainibe R. (2023). Occurrence and Molecular Characterization of Cryptosporidium Infection in HIV/Aids Patients in Algeria. Viruses, 15(2), 362.

Publication 2023

Abdominal pain Aids Animals Auramine Blood cell counts Cryptosporidium Diarrhoea Drug Faecal Farm animals Fluorescence Haart Hiv positive Inpatient Light Microscopy Nausea Oocysts Outpatient care Patients Pets Physicians Regimens

Corresponding Organization : Centre Hospitalier Universitaire de Rouen

Other organizations : Centre Hospitalo-Universitaire Bab El Oued, Centre de Recherche Nucléaire d’Alger

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Variable analysis

independent variables

Contact with animals (pets and farm animals)
Drinking water sources

dependent variables

Diarrhoea
Weight loss
Vomiting
Abdominal pain
Nausea
Types of HAART drug regimens
Blood CD4+ T-cell counts
Positive for Cryptosporidium

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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