Quantitative Cell-Cell Fusion Assay
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Corresponding Organization :
Other organizations : King Faisal University, Kafrelsheikh University, The University of Tokyo, National Institute of Infectious Diseases, Gifu University, Hallym University
Protocol cited in 1 other protocol
Variable analysis
- Compounds (dissolved in DMSO)
- Renilla luciferase (RL) activity
- Cell types (effector cells stably expressing DSP8-11 and S-protein, target cells stably expressing DSP1-7, CD26, and TMPRSS2)
- EnduRen (Renilla luciferase substrate) treatment
- Cell number (1.5x10^4 effector and target cells per well)
- Incubation time (4 hours)
- Incubation temperature (37°C)
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