Berberine (633-65-8) was purchased from Chengdu Puth Biotechnology (China) with a purity of more than 98%. We also used: fetal bovine serum (FBS) (L20711 BI Transgen biotech, Australia); Dulbecco’s Modified Eagle Medium (DMEM) (2049231, BIOIND, Israel); Cell Counting Kit-8 (CCK-8) (DCM7126, Beijing Lambolid Trading, China); lipopolysaccharide (12190801, Beijing Trading, China); and TRIzol reagent (182806, Semel Fishi Technology, USA). MMP-1 (L2104850), MMP-3 (L201014164), RANKL (L201216066), and TNF-α (L201215056) were all purchased from Wuhan Yunkun Technology (China). We also used one-step removal of premixed reagent for first chain synthesis of genomic cDNA (U9126, Tiangen biochemical Technology, China) and PerfectStart Green qPCR SuperMix (+ Dye Ⅰ /+ Dye Ⅱ) (O10529, Beijing full Gold Biotechnology, China) in the RT-qPCR. Phosphorylase inhibitor (TargetMol, USA), bicinchoninic acid (BCA) protein concentration determination kit (Beijing Soleibao Technology, China), Oncogene homologue (GTPase Hras, HRAS) antibody (Beijing Boosen Biotechnology, China), cyclic adenine ribonucleotide dependent transcription factor 2 (ATF-2) antibody (Affinity, China), p-ATF-2 antibody (Affinity), p38 antibody (Beijing Boosen Biotechnology), p-p38 antibody (Affinity), MAPK1/2 antibody (Affinity); p-MAPK1/2 antibody (Affinity), FOXO3 antibody (Affinity), p-FOXO3 antibody (Affinity), HIF-1 α Antibody (Affinity), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Proteintech, China), and sheep anti-rabbit-peroxidase (Beijing Boosen Biotechnology) were used in the western blot experiment.