Ishikawa and HEC1-A cells were selected for cell transfection. Short hairpin RNAs for circSMAD2 (sh-circSMAD2#1, sh-circSMAD2#2, sh-circSMAD2#3) or the control sh-NC were constructed by Invitrogen (Carlsbad, CA, USA). MiR-1277-5p mimics, miR-1277-5p inhibitors, siRNA of MFGE8, pcDNA-MFGE8 and their corresponding negative controls were obtained from Sangon (Shanghai, China). Then cell transfection was carried our using Lipofectamine 2000 (Invitrogen).
Protocol detail
Investigating circSMAD2 and miR-1277-5p in Cell Lines
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Corresponding Organization :
Other organizations : Shanxi Medical University
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Variable analysis
independent variables
- Sh-circSMAD2#1
- Sh-circSMAD2#2
- Sh-circSMAD2#3
- MiR-1277-5p mimics
- MiR-1277-5p inhibitors
- SiRNA of MFGE8
- PcDNA-MFGE8
- Not explicitly mentioned
- Sh-NC
- Corresponding negative controls for miR-1277-5p mimics, miR-1277-5p inhibitors, siRNA of MFGE8, and pcDNA-MFGE8
- Sh-NC (negative control for sh-circSMAD2#1, sh-circSMAD2#2, sh-circSMAD2#3)
- Corresponding negative controls for miR-1277-5p mimics, miR-1277-5p inhibitors, siRNA of MFGE8, and pcDNA-MFGE8
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