Total serum IgE level was determined using the Immulite XPI, solidphase chemiluminescence assay (Siemens Medical Solutions, Malvern, PA, USA). IgE levels to pork and beef were measured using a fluoroenzyme immunoassay (Phadia Immuno CAP; courtesy of Platts-Mills laboratory Charlottesville, VA, USA).3 (link) To measure specific IgE, for galactose alpha-1,3-galactose (alpha-gal) we used an assay which employs a monoclonal antibody to cetuximab with a correlation of 0.95 to the commercially available assay in Europe which uses an antibody to beef thyroglobulin.16 (link) Values greater than 0.35 IU/mL were considered positive in both assays. sTryp levels were determined using a commercial fluoro-enzyme immunoassay (Phadia Immuno CAP, Uppsala, Sweden) at CLIA-approved laboratories, and levels above 11.4 ng/mL were considered elevated.
Protocol detail
Measuring Serum IgE and Alpha-gal Levels
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Assays
Beef
Cetuximab
Chemiluminescence assay
Enzyme immunoassay
Galactose
Immunoassay
Monoclonal antibody
Pork
Serum
Thyroglobulin antibody
Corresponding Organization : National Institutes of Health
Other organizations : University of Virginia, University of Tennessee at Knoxville
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Variable analysis
independent variables
- Measurement of total serum IgE level using the Immulite XPI, solid-phase chemiluminescence assay
- Measurement of IgE levels to pork and beef using a fluoroenzyme immunoassay (Phadia Immuno CAP)
- Measurement of specific IgE for galactose alpha-1,3-galactose (alpha-gal) using an assay with a monoclonal antibody to cetuximab
- Measurement of sTryp levels using a commercial fluoro-enzyme immunoassay (Phadia Immuno CAP)
- Total serum IgE level
- IgE levels to pork and beef
- Specific IgE for galactose alpha-1,3-galactose (alpha-gal)
- STryp levels
- Positive control: Commercially available assay in Europe using an antibody to beef thyroglobulin for measurement of specific IgE for galactose alpha-1,3-galactose (alpha-gal)
- Negative control: Not explicitly mentioned
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