Regulation of ERK1/2 and Akt in RPE Cells

RPE cells (1 x 10⁵), grown in F12 with 10% FBS for 24 h, were transfected with miR-182 or negative control. Western blot analyses were performed as previously reported [22 (link)]. Antibodies for total ERK1/2, phosphorylated-ERK1/2, total Akt, phosphorylated-Akt, phosphorylated-Rb, c-Met and phosphorylated-Met were from Cell Signaling Technology (Beverly, MA).

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Wang L., Dong F., Reinach P.S., He D., Zhao X., Chen X., Hu D.N, & Yan D. (2016). MicroRNA-182 Suppresses HGF/SF-Induced Increases in Retinal Pigment Epithelial Cell Proliferation and Migration through Targeting c-Met. PLoS ONE, 11(12), e0167684.

Publication 2016

phosphorylated-Akt phosphorylated-Rb c-Met phosphorylated-Met

Antibodies C met Cells Erk1 Western blot analyses

Corresponding Organization : New York Eye and Ear Infirmary

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Variable analysis

independent variables

MiR-182 transfection
Negative control transfection

dependent variables

Total ERK1/2
Phosphorylated-ERK1/2
Total Akt
Phosphorylated-Akt
Phosphorylated-Rb
C-Met
Phosphorylated-Met

control variables

RPE cells (1 x 10^5)
F12 medium with 10% FBS
24 h growth period

controls

Negative control transfection

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