Hand-cut cross-sections were made from the base of the stem of both the inoculated and mock-treated cotton plants at 14 d after treatment. Lignin histochemistry was examined using Wiesner reagent (Pomar et al., 2004 ). Cross-sections were incubated for 10 min in a phloroglucinol solution (2% in 95% ethanol) or 95% ethanol (staining control), treated with 18% HCl for 5 min, and directly observed with a bright-field microscope using a Leica fluorescence microscope (DM2500, Leica, Wetzlar, Germany).
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Lignin Histochemistry in Cotton Stems
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Other organizations : Huazhong Agricultural University
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Variable analysis
independent variables
- Inoculation treatment (inoculated vs. mock-treated)
- Lignin histochemistry (examined using Wiesner reagent)
- Time after treatment (14 days)
- Plant part (base of the stem)
- Staining procedure (10 min incubation in phloroglucinol solution or 95% ethanol, 5 min treatment with 18% HCl)
- Microscope (Leica fluorescence microscope DM2500)
- Positive control: Phloroglucinol staining
- Negative control: 95% ethanol staining
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