HPLC-UV DNA Quantification Protocol
Corresponding Organization :
Other organizations : Beijing University of Technology
Variable analysis
- Concentration of DNA
- Amount of DNA
- HPLC-UV analysis
- Enzymatic hydrolysate of DNA
- Luna C18 column (4.6×250 mm, 5 μm in particle size)
- Gradient elution using deionized water (solvent A) and methanol (solvent B) at a flow rate of 0.7 mL/min
- UV signal monitored at 254 nm
- Solvent composition began at 5% B followed by linear increase to 22% B over 15 min, then raised to 80% over 2 min, and brought back to 5% over 2 min
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