Quantifying Scaffold Roughness and Mechanics

The roughness of the scaffolds surface was evaluated using an AFM (TAP150A) in the tapping mode (spring constant 2.919 n/m) as described previously (n=3).11 (link),12 (link) In brief, the root mean square roughness was calculated from the 5 µm scan of three areas using the NanoScope^® analysis software version 1.40 (Bruker Corporation, Billerica, MA, USA). The PeakForce quantitative nanomechanical mapping was used to obtain an FV modulus using the modified Hertzian model.

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Griffin M., Palgrave R., Baldovino-Medrano V.G., Butler P.E, & Kalaskar D.M. (2018). Argon plasma improves the tissue integration and angiogenesis of subcutaneous implants by modifying surface chemistry and topography. International Journal of Nanomedicine, 13, 6123-6141.

Publication 2018

TAP150A

Root Scan

Corresponding Organization : Royal National Orthopaedic Hospital

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Variable analysis

independent variables

Roughness of the scaffolds surface

dependent variables

Root mean square roughness
FV modulus

control variables

Tapping mode (spring constant 2.919 n/m)
5 µm scan of three areas
NanoScope® analysis software version 1.40

controls

Positive control: Not mentioned
Negative control: Not mentioned

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