Composition proportions of Xiaoyaosan prescription: Poria : Radix Paeoniae Alba : Radix Et Rhizoma Glycyrrhizae : Radix Bupleuri : Radix Angelicae Sinensis : Rhizoma Atractylodis Macrocephalae : Herba Menthae Haplocalycis : Rhizoma Zingiberis Recens equaled to 3 : 3 : 1.5 : 3 : 3 : 3 : 1 : 1. All 8 crude drugs were purchased from Beijing Tongrentang (Bozhou) Decoction Pieces Limited Company and authenticated by Dr. B. Liu, Department of Botany, and Beijing University of Chinese Medicine. All crude drugs were extracted by the Chinese medicine preparation room of China-Japan Friendship Hospital as previously described [7 (link)]. Extraction steps were performed as follows: Poria, Radix Paeoniae Alba, and Radix Rhizoma Glycyrrhizae were boiled and extracted three times with 10 volumes (2 h), 8 volumes (1 h), and 8 volumes (1 h) of water to obtain the extraction liquid (A). Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Atractylodis Macrocephalae, Herba Menthae, and Rhizoma Zingiberis Recens were soaked with 10 volumes of water for 12 h to obtain the volatile oil, drug liquid (B), and drug residue (C). Subsequently, C was boiled in 8 volumes of water for 1 h and extracted twice to obtain the extraction solution (D). Extraction solutions A, B, and D were mixed to form the water extraction liquid (E). Next, E was filtered and centrifuged (3000 r/min for 40 min). The supernatant was collected and vacuum dried at 70°C. Then, the dried product and the volatile oil were processed into dry decoction for use. The extraction rate was 18.8%. Xiaoyaosan (was dissolved in deionized water and administered by intragastric injection at a dose of) was 3.854 g/kg·d, and deionized water was used in all groups.
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