Anticonvulsant Screening in Zebrafish

The following anticonvulsants: carbamazepine (CBZ), ethosuximide (ETS), oxcarbazepine (OXC), lamotrigine (LTG), levetiracetam (LVT), zonisamide (ZSM), primidone (PMD), topiramate (TPR), and aspirin (ASP; negative control) were purchased from Sigma. Other AEDs used in this study were: diazepam (DZP; Roche), gabapentin (GBP; Fluka), tiagabine (TGB; Chemos), sodium valproate (VPA; Sanofi-Aventis), and phenytoin (PHT; Acros). All compounds were dissolved in DMSO and diluted in embryo medium to achieve a final DMSO concentration of 1% w/v. Embryo medium prepared with DMSO to a final concentration of 1% w/v served as a vehicle control (VHC). Pentylenetetrazol was also purchased from Sigma and was dissolved to 40 mM (2x stock) in embryo medium.