DNA was extracted from the frozen tissue and urine samples using the AllPrep DNA Mini kit (Qiagen, Inc., Valencia, CA, USA) according to the manufacturer's protocol. The concentration and purity of DNA were assessed by determining the OD260/OD280 ratio using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA). DNA was treated by bisulfite using an EZ-96 DNA Methylation-Gold™ kit (Zymo Research, Irvine, CA, USA) according to the manufacturer's protocol, as well as the previously described protocol (20 (link)), and stored at −80°C until use.
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DNA Extraction and Bisulfite Conversion
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Corresponding Organization :
Other organizations : Fujian Medical University, Quanzhou Normal University, People's Liberation Army No. 150 Hospital
Top 5 similar protocols
Variable analysis
independent variables
- DNA extraction method using the AllPrep DNA Mini kit
- Bisulfite treatment using the EZ-96 DNA Methylation-Gold™ kit
- DNA concentration
- DNA purity (OD260/OD280 ratio)
- Frozen tissue and urine samples
- Manufacturer's protocols for DNA extraction and bisulfite treatment
- Previously described protocol for bisulfite treatment (20)
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