Maintenance of Toxoplasma gondii Strains in Mouse Models

T. gondii GT1 strains were maintained in female BALB/c obtained from KOA Tech (Pyeogtaek, South Korea) as described previously (5 (link), 6 (link), 12 (link), 13 (link)). T. gondii GT1 and ME49 strains were maintained by serial intraperitoneal passage (GT1) or oral passage (ME49) in BALB/c mice as described (5 (link)). Spodoptera frugiperda Sf9 cells used for production of recombinant baculovirus (rBV) and virus-like particles were maintained in serum-free SF900 II medium (Invitrogen, Carlsbad, CA, USA) in spinner flasks at 27°C and 130–140 rpm. T. gondii (ME49)–infected sera from mice were used. Horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin IgG was purchased from Southern Biotech (Birmingham, AL, USA). Monoclonal mouse anti-M1 antibody was purchased from Abcam (Cambridge, UK).

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Lee S.H., Kang H.J., Lee D.H, & Quan F.S. (2019). Protective Immunity Induced by Incorporating Multiple Antigenic Proteins of Toxoplasma gondii Into Influenza Virus-Like Particles. Frontiers in Immunology, 9, 3073.

Publication 2018

BALB/c SF900 II medium Monoclonal mouse anti-M1 antibody

Anti igg Baculovirus Balb c mice Female Goat Horseradish peroxidase Immunoglobulin Monoclonal antibody Mouse Sera Sf9 cells Spodoptera frugiperda Strains Virus particles

Corresponding Organization : Kyung Hee University Medical Center

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Variable analysis

independent variables

T. gondii GT1 strains
T. gondii GT1 and ME49 strains

dependent variables

Not explicitly mentioned

control variables

Female BALB/c mice obtained from KOA Tech (Pyeogtaek, South Korea)
Spodoptera frugiperda Sf9 cells used for production of recombinant baculovirus (rBV) and virus-like particles
Horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin IgG
Monoclonal mouse anti-M1 antibody

controls

Positive control: T. gondii (ME49)–infected sera from mice
Negative control: not explicitly mentioned

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