Adult Cuticle Imaging and Wing Disc Immunostaining
Corresponding Organization : University of North Carolina at Chapel Hill
Variable analysis
- Mounting adult cuticles on glass slides in Hoyers
- Performing wing imaginal disc immunostaining
- Imaging the mounted adult cuticles using a digital camera mounted on a stereo-dissecting scope
- Performing confocal microscopy on the wing imaginal disc immunostaining
- Concentration of mouse anti-Ubx antibody used (1:30 dilution)
- Positive control: Not specified
- Negative control: Not specified
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