Glucose Modulation of Feeding Behavior in Flies

Flies were immobilized as previously described.^{17 (link)} Approximately 50 nL of AHL buffer (108 mM NaCl, 8.2 mM MgCl₂, 4 mM NaHCO₃, 1 mM NaH₂PO₄, 2 mM CaCl₂, 5 mM KCl, 5 mM HEPES)^{20 (link)} with or without the addition of 100 mM D-glucose was injected into the thorax of these flies with a glass micropipette. The glass micropipette was pulled from thick-walled borosilicate capillaries (Sutter Instruments, BF120-69-10) with a micromanipulator (Sutter Instruments, MP225) to control its movements. Flies’ food consumption was measured immediately after AHL injection by the MAFE assay.

Free full text: Click here

Qi W., Wang G, & Wang L. (2020). A novel satiety sensor detects circulating glucose and suppresses food consumption via insulin-producing cells in Drosophila. Cell Research, 31(5), 580-588.

Publication 2020

BF120-69-10 MP225

Assay Buffer Capillaries Flies Food Glucose Hepes Mgcl2 Movements Nacl Nahco3 Thorax

Corresponding Organization :

Other organizations : Zhejiang University

Top 5 similar protocols

Variable analysis

independent variables

Addition of 100 mM D-glucose to the AHL buffer

dependent variables

Flies' food consumption measured immediately after AHL injection by the MAFE assay

control variables

Flies immobilized as previously described
Approximately 50 nL of AHL buffer (108 mM NaCl, 8.2 mM MgCl2, 4 mM NaHCO3, 1 mM NaH2PO4, 2 mM CaCl2, 5 mM KCl, 5 mM HEPES) injected into the thorax of the flies
Glass micropipette pulled from thick-walled borosilicate capillaries (Sutter Instruments, BF120-69-10) with a micromanipulator (Sutter Instruments, MP225) to control its movements

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!