Genotyping Four LMO1 Gene SNPs

About 2 mL of peripheral blood was collected from each subject for genotyping. Four LMO1 gene SNPs (rs110419 A>G, rs4758051 G>A, rs10840002 A>G and rs204938 A>G) identified in a GWAS on neuroblastoma were chosen for genotyping [29 (link)]. Genomic DNA was isolated from peripheral blood leukocytes with a TIANamp Blood DNA Kit (TianGen Biotech, Beijing, China) [38 (link), 40 (link)]. A 7900 Sequence Detection System (Applied Biosystems, Foster City, CA) and Taqman real-time PCR were used to genotype the LMO1 SNPs, as described thoroughly elsewhere [44 (link), 45 (link)]. To obtain convincing results, we performed the genotyping blindly, not knowing whether each subject was a case or control. We also randomly selected 10% of the samples for repeated genotyping, and the genotype concordance was 100%.

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Liu G.C., Zhuo Z.J., Zhu S.B., Zhu J., Jia W., Zhao Z., Hu J.H., He J., Wang F.H, & Fu W. (2017). Associations between LMO1 gene polymorphisms and Wilms' tumor susceptibility. Oncotarget, 8(31), 50665-50672.

Publication 2017

TIANamp Blood DNA Kit 7900 Sequence Detection System Taqman real-time PCR

Blood Gene Genomic Genotype Gwas Lmo1 Neuroblastoma Peripheral blood leukocytes Real time pcr Snps

Corresponding Organization :

Other organizations : Guangzhou Medical University, Chinese University of Hong Kong, Harbin Medical University, Third Affiliated Hospital of Harbin Medical University

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Variable analysis

independent variables

4 LMO1 gene SNPs (rs110419 A>G, rs4758051 G>A, rs10840002 A>G and rs204938 A>G)

dependent variables

Genotyping results for the 4 LMO1 gene SNPs

control variables

Peripheral blood samples collected from each subject
Genomic DNA isolated from peripheral blood leukocytes using a TIANamp Blood DNA Kit
Genotyping performed using a 7900 Sequence Detection System and Taqman real-time PCR
Genotyping performed blindly, without knowing whether each subject was a case or control
10% of the samples were randomly selected for repeated genotyping, with 100% genotype concordance

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