Quantitative Analysis of DlCDPs

Total RNA was isolated from germination buds and dormant buds using RNA prep Pure Plant Kit (Tiangen, Beijing, China), and reverse transcribed into cDNA using PrimeScript™ RT reagent Kit (Perfect Real Time, Takara, Japan). Quantitative real-time PCR analysis of 8 selected DlCDPs was performed on a LightCycler480 instrument (Roche) using Taq Pro Universal SYBR qPCR Master Mix SYBR Green premix Ex Taq Kit (TaKaRa, Dalian, China) on an Applied Biosystems 7500 Real-Time System (Applied Biosystems, Foster City, CA, USA). The q-PCR amplification program was: 95°C pre-denaturation for 5 seconds, 95°C denaturation for 30 seconds, 60°C annealing for 30 seconds; 72°C extension for 30 seconds, 40 cycles. GAPDH gene was used as an internal reference gene (Liu et al., 2014 (link)), and the relative expression of each gene was calculated using the 2-ΔΔCT method. The primers used in this study are shown in Table S5.