The strains and plasmids used in this study were listed in Table 2. Bacillus subtilis 168 and E. coli were grown on Luria-Bertani (LB) medium. When needed, antibiotics were added to the medium at the following concentrations: ampicillin, 100 μg/ml; kanamycin, 50 μg/ml. All media were sterilized by autoclaving at 121 °C for 20 min.

The strains and plasmids used in this study.

Strain or plasmidSource or reference
strains
 Bacillus subtilis 168ATCC 6051
 E. coli DH5αTransGen Biotech
 E. coli BL21(DE3)TransGen Biotech
 E. coli BL21(DE3)-BsAcsAIn this work
 E. coli BL21(DE3)-BsAcuAIn this work
 Salmonella enterica Δacs (JE7758)18 (link)
plasmids
  pET-28aThermo Scientific
  pBAD3018 (link)
  pGEX-4T-2Thermo Scientific
  pET-BsAcsALab stock
  pGEX-BsAcuAIn this work
The antibodies and columns for purification of proteins used were as follows: anti-acetyl-lysine antibody (ImmuneChem Pharmaceuticals, Burnaby, CA); pan anti-propionyl-lysine antibody (PTM Biolabs); HRP conjugates (anti-PrK; ImmuneChem); glutathione transferase (GST) agarose column and nickel-nitrilotriacetic acid (Ni-NTA) superflow column (Qiangen, Valencia, CA).
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