Abiotic Stress Response Profiling in Arabidopsis

The RNA-Seq was performed on samples from 10-day-old whole seedlings and roots. Whole seedlings were treated with 150 mM NaCl for 1, 2, and 4 h or 300 mM mannitol for 1, 2, and 4 h each. Subsequently, the samples were harvested, and either complete (whole seedlings) or fractionated (roots only) samples were used for RNA isolation. After total RNA isolation, 2 μg of each sample was mixed and used for mRNA-Seq library preparation, which was performed by E-biogen (https://www.e-biogen.com, accessed on 13 September 2022), as previously described [51 (link)]. High-throughput paired-end 100 bp sequencing was performed using a HiSeq X10 system (Illumina, Inc., San Diego, CA, USA). Two biological replicates of each sample were used for RNA-Seq.