Whole Blood Immunophenotyping Protocol

Whole blood collected immediately before the first VSV gag administration and then 1 and 7 days after was stained in TruCOUNT tubes as previously described (Elizaga et al., 2018 (link); Hensley et al., 2012 ; Hensley-McBain et al., 2014 (link)) using the following antibody staining panel: (antibodies from BD Biosciences, unless otherwise indicated): CD14–V450, CD19–V450, CD45–AmCyan, CD4–FITC, CD8–PerCP-Cy5.5, CD123–PE, HLA-DR–ECD (Beckman Coulter), CD86–PE-Cy5, CD56–PE-Cy7, CD11c–APC, CD3–Alexa700 and CD16–APC-Cy7. We used these measurements in Figures 6G and 6H to validate changes in cell type abundance that were detected by scRNA-seq.