Chicken Hypothalamic Neuropeptide Expression
Corresponding Organization : University of Arkansas at Fayetteville
Other organizations : University of Maryland, College Park, United States Department of Agriculture, Agricultural Research Service
Protocol cited in 3 other protocols
Variable analysis
- None explicitly mentioned
- Relative expression levels of the following genes measured by real-time qPCR:
- Neuropeptide Y (NPY)
- Agouti-related peptide (AgRP)
- Proopiomelanocortin (POMC)
- Cocaine and amphetamine regulated transcript (CART)
- Orexin (ORX)
- Orexin receptor 1/2 (ORXR1/2)
- Leptin receptor (Ob-R)
- HSP 60, 70
- Heat shock factor 1-4 (HSF1-4)
- AMP-activated protein kinase alpha 1/2 (AMPKα1/2)
- Mechanistic target of rapamycin (mTOR)
- P70 ribosomal protein S6 kinase 1 (S6K1)
- HSP90
- Tissue source: Chicken hypothalamus
- RNA extraction method: Trizol reagent
- DNAse treatment and reverse transcription of RNA
- Assessment of RNA integrity and quality using agarose gel electrophoresis and RNA concentration/purity using Take three micro volume plate and Synergy HT multi-mode microplate reader
- Real-time qPCR assay using SYBR Green Master Mix and Applied Biosystems 7500 Real-Time PCR system
- Housekeeping gene: Ribosomal 18S
- None explicitly mentioned
- No RT products used as templates in the qPCR
Annotations
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